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Section of Viral Infections

Research group:   SA Professor   Naokazu Takeda, Ph.D.
    SA Researcher   Natsuko Kishishita, Ph.D.
    Postdoctoral Fellow   Uamporn Siripanyaphinyo, Ph.D.
    Postdoctoral Fellow   Sompong Sapsutthipas, Ph.D.
    Postdoctoral Fellow   Nitchakarn Noranate, Ph.D.
    Assistant Researcher   Piraporn Utachee, M.Sc.
    Assistant Researcher   Chris Verathamjamras, M.Sc.
    Assistant Researcher   Uranan Tumkosit, M.Sc.
   
   

　The Section of viral Infections focuses on two emerging and reemerging viral diseases that are prevalent in Asian countries including Thailand in collaboration with the National Institute of Health, Department of Medical Sciences, Ministry of Public Health of Thailand. One is mosquito-borne infectious diseases, and we study chikungunya fever from epidemiological, molecular biological, and immunological aspects. The other is blood-borne infectious diseases (HIV diseases/AIDS), and we perform virological and immunological characterization of the HIV-1 CRF01_AE strains prevalent in Southeast Asian countries including Thailand. In addition, the studies to elucidate the mechanism of the anti-retroviral drug resistance is in progress.





Recent publications:
1. Boonchawalit, S., Jullaksorn, D., Uttiyoung, J., Yowang, A., Krathong, N., Chautrakul, S., Yamashita, A., Ikuta, K., Roobsoong, A., Kanitvittaya, S., Sawanpanyalert, P. and Kameoka, M. (2011) Molecular evolution of HIV-1 CRF01_AE Env in Thai patients. PLoS ONE 6, e27098.

2. Sapsutthipas, S., Kitagawa, Y., Tokunaga, K., Ikuta, K. and Kameoka, M. (2011) Viral factors involved in adapter-related protein complex 2 alpha 1 subunit-mediated regulation of human immunodeficiency virus type 1 replication. Southeast Asian J. Trop. Med. Public Health 42, 311-319.

3. Isarangkura-na-ayuthaya, P., Kaewnoo, W., Auwanit, W., de Silva, U. C., Ikuta, K., Sawanpanyalert, P. and Kameoka, M. (2010) Appearance of drug resistance-associated mutations in human immunodeficiency virus type 1 CRF01_AE integrase derived from drug-naive Thai patients. AIDS Res. Hum. Retroviruses 26, 1341-1343.

4. Kanai, Y., Chittaganpitch, M., Nakamura, I., Li, G. M., Bai, G. R., Li Y. G., Ikuta, K. and Sawanpanyalert, P. (2010) Distinct propagation efficiencies of H5N1 influenza virus Thai isolates in newly established murine respiratory region-derived cell clones. Virus Res. 153, 218-225.

5. Soonthornsata, B., Tian, Y.-S., Utachee, P., Sapsutthipas, S., Isarangkura-na-ayuthaya, P., Auwanit, W., Takagi, T., Ikuta, K., Sawanpanyalert, P., Kawashita, N. and Kameoka, M. (2010) Design and evaluation of antiretroviral peptides corresponding to the C-terminal heptad repeat region (C-HR) of human immunodeficiency virus type 1 envelope glycoprotein gp41. Virology 405, 157-164.

6. Kameoka, M., Isarangkura-na-ayuthaya, P., Kameoka, Y., Sapsutthipas, S, Soonthornsata, B., Nakamura, S., Tokunaga, K., Sawanpanyalert, P., Ikuta, K. and Auwanit, W. (2010) The role of lysine residue at amino acid position 165 of human immunodeficiency virus type 1 CRF01_AE Gag in reducing viral drug susceptibility to protease inhibitors. Virology 405, 129-138.

7. Kanai, Y., Boonsathorn, N., Chittaganpitch, M., Bai, G., Li, Y., Kase, T., Takahashi, K., Okuno, Y., Jampangern, W., Ikuta, K. and Sawanpanyalert P. (2010) The impact of antigenic drift of influenza A virus on human herd immunity: Sero-epidemiological study of H1N1 in healthy Thai population in 2009. Vaccine 28, 5437-5444.

8. Li, Y. G., Chittaganpitch, M., Waicharoen, S., Kanai, Y., Bai, G. R., Warachit, J., Kameoka, M., Takeda, N., Ikuta, K. and Sawanpanyalert, P. (2010) Characterization of H5N1 influenza viruses in vitro isolated from humans. Virol. J. 7, 112.

9. Utachee, P., Nakamura, S., Isarangkura-na-ayuthaya, P., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., Auwanit, W. and Kameoka, M. (2010) N-linked glycosylation sites in V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 regulates viral neutralization susceptibility to the human monoclonal antibody specific for CD4 binding domain. J. Virol. 84, 4311-4320.

10. Jullaksorn, D., Boonchawalit, T., Uttiyoung, J., Soonthornsata, B., Yowang, A., Krathong, N., Chautrakul, S., Ikuta, K., Roobsoong, A., Kanitvittaya, S., Sawanpanyalert, P. and Kameoka, M. (2010) Sustained appearance of drug resistance-associated mutations in CRF01_AE protease and reverse transcriptase derived from protease inhibitor-naive Thai patients. Southeast Asian J. Trop. Med. Public Health 41, 347-357.

11. Pongsuwannna, Y., Guntapong, R., Tacharoenmuang, R., Prapanpoj, M., Kameoka, M. and Taniguchi, K. (2009) A long-term survey on the distribution of the human rotavirus G type in Thailand. J Med Virol 82, 157-163.

12. Bai, G. R., Chittaganpitch, M., Kanai, Y., Li, Y. G., Auwanit, W., Ikuta, K. and Sawanpanyalert, P. (2009) Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand. Biochem Biophys Res Commun 390, 897-901.

13. Auwanit, W., Isarangkura-Na-Ayuthaya, P., Kasornpikul, D., Ikuta, K., Sawanpanyalert, P. and Kameoka, M. (2009) Detection of drug resistance-associated and background mutations in human immunodeficiency virus type 1 CRF01_AE protease and reverse transcriptase derived from drug treatment-naive patients residing in central Thailand. AIDS Res Hum Retroviruses 25, 625-631.

14. Kameoka, M., Kameoka, Y., Utachee, P., Kurosu, T., Sawanpanyalert, P., Ikuta, K. and Auwanit, W. (2009) RNA Interference Directed against Axin1 Upregulates Human Immunodeficiency Virus Type 1 Gene Expression by Activating the Wnt Signaling Pathway in HeLa-Derived J111 Cell. AIDS Res Hum Retroviruses.

15. Jinnopat, P., Isarangkura-Na-Ayuthaya, P., Utachee, P., Kitagawa, Y., de Silva, U. C., Siripanyaphinyo, U., Kameoka, Y., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., Auwanit, W. and Kameoka, M. (2009) Impact of Amino Acid Variations in Gag and Protease of Human Immunodeficiency Virus Type 1 CRF01_AE Strains on Drug Susceptibility of Virus to Protease Inhibitors. J Acquir Immune Defic Syndr.

16. Utachee, P., Jinnopat, P., Isarangkura-Na-Ayuthaya, P., de Silva, U. C., Nakamura, S., Siripanyaphinyo, U., Wichukchinda, N., Tokunaga, K., Yasunaga, T., Sawanpanyalert, P., Ikuta, K., Auwanit, W. and Kameoka, M. (2009) Phenotypic studies on recombinant human immunodeficiency virus type 1 (HIV-1) containing CRF01_AE env gene derived from HIV-1-infected patient, residing in central Thailand. Microbes Infect 11, 334-343.

17. Utachee, P., Jinnopat, P., Isarangkura-Na-Ayuthaya, P., de Silva, U. C., Nakamura, S., Siripanyaphinyo, U., Wichukchinda, N., Tokunaga, K., Yasunaga, T., Sawanpanyalert, P., Ikuta, K., Auwanit, W. and Kameoka, M. (2009) Genotypic characterization of CRF01_AE env genes derived from human immunodeficiency virus type 1-infected patients residing in central Thailand. AIDS Res Hum Retroviruses 25, 229-236.

18. Li, Y. G., Thawatsupha, P., Chittaganpitch, M., Rungrojcharoenkit, K., Li, G. M., Nakaya, T., Auwanit, W., Ikuta, K. and Sawanpanyalert, P. (2008) Higher in vitro susceptibility of human T cells to H5N1 than H1N1 influenza viruses. Biochem Biophys Res Commun 371, 484-489.

19. Kitagawa, Y., Kameoka, M., Shoji-Kawata, S., Iwabu, Y., Mizuta, H., Tokunaga, K., Fujino, M., Natori, Y., Yura, Y. and Ikuta, K. (2008) Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome. Virology 373, 171-180.

20. Kurosu, T., Chaichana, P., Yamate, M., Anantapreecha, S. and Ikuta, K. (2007) Secreted complement regulatory protein clusterin interacts with dengue virus nonstructural protein 1. Biochem Biophys Res Commun 362, 1051-1056.

21. Kameoka, M., Kitagawa, Y., Utachee, P., Jinnopat, P., Dhepakson, P., Isarangkura-na-ayuthaya, P., Tokunaga, K., Sato, H., Komano, J., Yamamoto, N., Oguchi, S., Natori, Y. and Ikuta, K. (2007) Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes. Biochem Biophys Res Commun 359, 729-734.