|
|
| Section of Bacterial Infections |
| Research group: |
|
SA Professor |
|
Shigeyuki Hamada, D.D.S., Ph.D. |
| |
|
SA Associate Professor |
|
Yumi Kumagai, Ph.D. |
| |
|
SA Assistant Professor |
|
Kazuhisa Okada, Ph.D. |
| |
|
Assistant Researcher |
|
Chetsada Boonthimat, M.Sc. |
| |
|
Assistant Researcher |
|
Wirongrong Natakuathung, M.Sc. |
The Section of Bacterial Infections pay special attention to a variety
of bacterial infectious diseases that are prevalent or break out in Asian
countries, including the Kingdom of Thailand and Japan. These studies are
performed in collaboration with researchers at the National Institute of
Health, Department of Medical Sciences, Ministry of Public Health of Thailand.
In the Program of Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) during the 2010 to 2014 fiscal years, we give the highest priority to cholera, an important enteric infectious diseases in Thailand and Vibrio cholerae. Our recent studies have revealed the emergence, year-long survival, or
disappearance of a particular type (s) of V. cholerae O1 in terms of spatial and temporal aspects. In addition, genomic and
molecular characterization of unique isolates of V. cholerae O1 is now in progress.
Streptococcus suis, which is occasionally pathogenic and frequently isolated from diseased
pigs, has been recognized to induce systemic (zoonotic) infections in humans,
mainly in Asian countries, including northeastern Thailand. Attempts are
being made to develop a diagnostic kit to detect S. suis based on the isothermal gene amplification method. We will also try to
elucidate its pathogenic mechanisms.
Group A streptococcus (GAS) is well known to induce self-limited pharyngitis
to life-threatening invasive infections in humans who live in various parts
of the world. In Thailand, however, epidemiological information regarding
GAS is definitely lacking. Thus, we examine the distribution of emm types and superantigen genes of Thai isolates of GAS with special reference
to the disease pattern.
Recent publications:
1. Okada K, Roobthaisong A, Nakagawa I, Hamada S, Chantaroj S. Genotypic
and PFGE/MLVA analyses of Vibrio cholerae O1: geographical spread and temporal changes of isolates during the 2007-2010
cholera outbreaks in Thailand. PLoS One 2012;7:e30863.
2. Takeuchi D, Kerdsin A, Pienpringam A, Loetthong P, Samerchea S, Luangsuk P, Khamisara K, Wongwan N, Areeratana P, Chiranairadul P, Lertchayanti S, Petcharat S, Yowang A, Chaiwongsaen P, Nakayama T, Akeda Y, Hamada S, Sawanpanyalert P, Dejsirilert S, Oishi K. Population-Based Study of Streptococcus suis Infection in Humans in Phayao Province in Northern Thailand. PLoS One 2012. 7(2):e31265.
3. Okada K, Chantaroj S, Roobthaisong A, Hamada S, Sawanpanyalert P. A
Cholera Outbreak of the Vibrio cholerae O1 El Tor Variant Carrying Classical CtxB in Northeastern Thailand in
2007. Am J Trop Med Hyg, 2010. 82(5) 875-878.
4. Puiprom O, Chantaroj S, Gangnonngiw W, Okada K, Honda T, Taniguchi T, Sawanpanyalert P. Identification of colonization factors of enterotoxigenic Escherichia coli with PCR-based technique. Epidemiol Infect 2010. 138(4):519-24.
5. Sakurai A, Maruyama F, Funao J, Nozawa T, Aikawa C, Okahashi N, Shintani
S, Hamada S, Ooshima T, Nakagawa I. Specific behaviour of intracellular
Streptococcus pyogenes that has undergone autophagic degradation is associated
with bacterial streptolysin O and host small G proteins Rab5 and Rab7.
J Biol Chem 2010. 285(29):22666-22675.
6. Aikawa C, Nozawa T, Maruyama F, Tsumoto K, Hamada S, Nakagawa I. Reactive
oxygen species induced by Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells. 2010.
Cell Microbiol 12(6):814-830.
7. Okada K, Chantaroj S, Taniguchi T, Suzuki Y, Roobthaisong A, Puiprom
O, Honda T, Sawanpanyalert P. A rapid, simple, and sensitive loop-mediated
isothermal amplification method to detect toxigenic Vibrio cholerae in rectal swab samples. Diagn Microbiol Infect Dis 2010. 66(2):135-9.
8. Maruyama F, Kobata M, Kurokawa K, Nishida K, Sakurai A, Nakano K, Nomura R, Kawabata S, Ooshima T, Nakai K, Hattori M, Hamada S, Nakagawa I. Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content. BMC Genomics 2009. 10:358.
9. Yamaguchi M, Minamide Y, Terao Y, Isoda R, Ogawa T, Yokota S, Hamada
S, Kawabata S. Nrc of Streptococcus pneumoniae suppresses capsular expression and enhances anti-phagocytosis. Biochem
Biophys Res Commun 2009. 390:155-160.
10. Okamoto S., Terao Y, Hasuike K, Hamada S, and Kawabata S. A novel streptococcal
leucine zipper protein (Lzp) binds to human immunoglobulins. Biochem. Biophys.
Res. Commun. 2008. 377(4):1128-34.
|
|
|
